Life Sciences in Space
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Four-year bacterial monitoring in the International Space Station - Japanese Experiment Module «Kibo» with culture-independent approach
// Space Research.
– 2016. – ¹ 1.
– P. 1 - 14.
DOI: 10.7256/2453-8817.2016.1.20495 URL: http://en. nbpublish.com/library_read_article.php?id=20495
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Studies on the relationships between humans and microbes in space habitation environments are critical for success in long-duration space missions, to reduce potential hazards to the crew and the spacecraft infrastructure. We performed microbial monitoring in the Japanese Experiment Module “Kibo”, a part of the International Space Station, for 4 years after its completion, and analyzed samples with modern molecular microbiological techniques. Sampling was performed in September 2009, February 2011, and October 2012. The surface of the incubator, inside the door of the incubator, an air intake, air diffuser, and handrail were selected as sampling sites. Sampling was performed using the optimized swabbing method. Abundance and phylogenetic affiliation of bacteria on the interior surfaces of Kibo were determined by quantitative PCR and pyrosequencing, respectively. Bacteria in the phyla Proteobacteria (γ-subclass) and Firmicutes were frequently detected on the interior surfaces in Kibo. Families Staphylococcaceae and Enterobacteriaceae were dominant. Most bacteria detected belonged to the human microbiota; thus, we suggest that the bacterial cells are transferred to the surfaces in Kibo from the astronauts. Environmental bacteria such as Legionella spp. were also detected. From the data on bacterial abundance and phylogenetic affiliation, Kibo has been microbiologically well maintained; however, the microbial community structure in Kibo may change with prolonged stay of astronauts. Continuous monitoring is required to obtain information on changes in the microbial community structure in Kibo.
Swabbing method, Bacterial monitoring, Kibo, International Space Station, Space Studies, Environmental Sciences, Microbiology, Space habitat, Bacterial virulence, Long space missions
Yamaguchi N. et al. Microbial monitoring of crewed habitats in space -Current status and future perspectives. Microbes Environ.29, 250–260 (2014).
Wilson J. W. et al. Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq. Proc. Natl Acad. Sci. USA; 104, 16299–16304 (2007).
Kim W. et al. Spaceflight promotes biofilm formation byPseudomonas aeruginosa. PLoS One 8, e62437 (2013).
Borchers, A. T., Keen, C. L. & Gershwin, M. E. Microgravity and immune responsiveness. Nutrition 18, 889–898 (2002).
Kawai, M., Yamaguchi, N. & Nasu, M. Rapid enumeration of physiologically active bacteria in purified water used in the pharmaceutical manufacturing process. J. Appl. Microbiol. 86, 496–504 (1999).
Yamaguchi, N., Ichijo, T., Sakotani, A., Baba, T. & Nasu, M. Global dispersion of bacterial cells on Asian dust. Sci. Rep. 2, 525 (2012).
Ichijo, T., Hieda, H., Ishihara, R., Yamaguchi, N. & Nasu, M.Bacterial monitoring with adhesive sheet in the International S